The CRP fluorescence immunochromatography quantitative detector
Yang Jianpeng1,2, Gao Yueming1,2, Wei Jianchong2,3, Chen Jianguo2,4, Jiang Haiyan2,4,5
1. College of Physics and Telecommunication Engineering, Fuzhou University, Fuzhou 350108; 2. Key Laboratory of Medical Instrumentation and Pharmaceutical Technology of Fujian Province, Fuzhou 350108; 3. College of electrics and information science, Fujian Jiangxia University, Fuzhou 350116; 4. College of Electrical Engineering and Automation, Fuzhou University, Fuzhou 350108; 5. Precision Machining Engineering Technology Research Center, Putian, Fujian 351100
Abstract：In this study, a set of fluorescence immunochromatographic quantitative detector for serum c-reactive protein (CRP) is developed. The image information of fluorescent strips is collected by using CMOS image sensor, and the image noise is filtered out by the method of mathematical morphology. The Otsu threshold segmentation algorithm is used to obtain the information of the image and to calculate the results. In this study, a number of different concentrations of fluorescence immunochromatographic strips are used to detect. The result shows that the CRP fluorescence immunochromatographic detector has good repeatability, and linearity. CV<3.4% and fit R2>99%. The detector has good performance and can accurately detect CRP value.
[1] Li Y P, Mold C, Du Clos T W. Sublytic complement attack exposes C-reactive protein binding sites on cell membranes[J]. Journal of Immunology (Baltimore, Md.: 1950), 1994, 152(6): 2995-3005. [2] 陈俊蕾, 张唯, 李新霞, 等. 背景荧光猝灭-免疫层析法检测C-反应蛋白[J]. 高等学校化学学报, 2018, 39(1): 41-47. [3] Moutachakkir M, Lamrani Hanchi A, Baraou A, et al.Immunoanalytical characteristics of C-reactive protein and high sensitivity C-reactive protein[J]. Annales de Biologie Clinique, 2017, 75(2): 225-229. [4] Dow C A, Templeton D L, Lincenberg G M, et al.Elevations in C-reactive protein and endothelin-1 system activity in humans[J]. Life Sciences, 2016, 159: 66-70. [5] 杨善敏, 刘少忠. 冠心病患者C反应蛋白测定的临床意义[J]. 安徽医学, 2009, 30(8): 937-938. [6] 姚振宇, 武一, 吉尚戎. CRP变构及其促炎机制研究进展[J]. 中国科学: 生命科学, 2017(8): 863-870. [7] Ghazavi A, Mosayebi G, Solhi H, et al.Serum markers of inflammation and oxidative stress in chronic opium (Taryak) smokers[J]. Immunology Letters, 2013, 153(1/2): 22-26. [8] Tagata K, Yokoyama S, Ginbo T, et al.Quantitative capillary reversed passive latex agglutination test for C-reactive protein (CRP) in the dog[J]. Veterinary Research Communications, 1996, 20(1): 21-30. [9] Zhang Xiaolu, Liu Juntian, Pang Xiaoming, et al.Eplerenone inhibits aldosterone-induced CRP Gener- ation in rat vascular smooth muscle cells by regulating the MR-ROS-ERK1/2 signal pathway[J]. European Journal of Inflammation, 2017, 15(3): 210-218. [10] 艾建勇, 金立军. 基于紫外图像的接触网棒瓷绝缘子污秽状态检测[J]. 电工技术学报, 2016, 31(10): 112-118. [11] 王欣, 孙鹏. 智能变电站中指针式仪表图像自动识别方法[J]. 电气技术, 2016, 17(5): 7-10. [12] 申洪明, 黄少锋, 费彬, 等. 基于数学形态学的换相失败检测新方法[J]. 电工技术学报, 2016, 31(4): 170-177. [13] 乔玲玲. 图像分割算法研究及实现[D]. 武汉: 武汉理工大学, 2009. [14] 周莉. 单相电能表自动检定流水线系统的应用研究[J]. 电气技术, 2014, 15(2): 59-63. [15] 林婉媚, 彭成丰. 血液生化指标CRP在临床上的检测意义[J]. 中国社区医师: 医学专业, 2010(20): 181.
2018, Vol. 19 
